BGS
Keyworth, Nottingham, UK, United Kingdom

STORAGE

TRANSPORT

Micro Lab (S16) (UK1.5)

BGS Geomicrobiology Laboratory

A fully equipped Containment Level 2 microbiology laboratory is available with the capability of handling geological materials (core materials and groundwaters). The Facility specialises in evaluating the impacts of CO2 injection (and associated impurities) on deep subsurface indigenous microbial populations and the effects of those organisms on the movement of CO2, solutes and contaminants and the potential for impacting CO2 storage.

Equipment and techniques include:

·         Microaerophilic/Anaerobic Chamber – a variable atmosphere workstation to study O2 sensitive microbes.

·         Molecular microbiology equipment - for DNA extraction and amplification (PCR) for identification of microbial isolates and characterisation of microbial communities (Denaturing Gradient Gel Electrophoresis and Oxford Nanopore DNA sequencing)

·         Deltatox analyser –for total viable biomass quantification using ATP analysis.

·         Biological Flow Apparatus – bespoke flow-through equipment investigating microbial effects on transport properties in intact/fractured rock cores under in-situ conditions.

·         Incubators for the cultivation and isolation of bacteria.

·         Epifluorescence microscopy for the assessment of bacterial numbers.

Applications include,

·         Environmental issues and the geological storage of carbon dioxide – extensive research has been undertaken in the UK and overseas into the effects of carbon dioxide leakage on marine and terrestrial ecosystems

·         The influences of microbes and biofilms on mass transport properties through geological media – using the biological flow apparatus

·         Microbial effects on mineral dissolution and precipitation (e.g. fracture and pathway sealing)

·         Groundwater quality, including transport and viability studies in groundwater systems

·         Development of imaging to assess distribution and enumeration of microorganisms in geological materials

Areas of research

nd

Installations

The Facility has a proven track record investigating the effects of CO2 leakage on ecosystems. For over 5 years it has conducted extensive research in both the UK and overseas and currently involved in projects assessing the ecosystem response to CO2 leakage and in understanding microbial activity in the reservoir.

The Facility can examine the impacts of CO2 injection on deep subsurface microbial populations and the impacts of those organisms on CO2 movement in the deep subsurface. Work in the field evaluates the environmental impacts of CO2 on surface ecosystems (e.g. botany) followed by laboratory based microbiology (e.g. DNA based methods, epifluorescence, microtox).

The Biological Flow Apparatus (BFA) can be modified to investigate interactions between microbes and injected CO2 in fractured or intact rock cores under realistic conditions of pressure and temperature.

State of the Art, uniqueness & specific advantages

The Facility has a proven track record investigating the effects of CO2 leakage on ecosystems.  For over 10 years it has conducted extensive research in both the UK and overseas including projects assessing the ecosystem response to CO2 leakage and in understanding various aspects of microbial activity under reservoir conditions.

The Facility can examine the impacts of CO2 injection on deep subsurface microbial populations and the impacts of those organisms on CO2 movement in the deep subsurface.  Work in the field evaluates the environmental impacts of CO2 on surface ecosystems followed by laboratory based microbiology (e.g. DNA based methods, epifluorescence, microtox).

The Biological Flow Apparatus (BFA) can be modified to investigate interactions between microbes and injected CO2 in fractured or intact rock cores under realistic conditions of pressure and temperature.

Scientific Environment

nd

Operating by

BGS

British Geological Survey, Natural Environment Research Council
United Kingdom
STORAGE technologies:
Reactivity/mineralisation, Leakage mitigation/remediation, Monitoring
TRANSPORT technologies:
CO2 pipeline transport and integrity
Research Fields:
Microbiology

Location & Contacts

Location
Keyworth, Nottingham, UK, United Kingdom
Contacts
Simon Gregory
RICC Contacts - Secondary contact
Audrey Ougier-Simonin

Facility Availability

Availability per year (in UA)
2 weeks
Present facility state of access
Partially Accessible
If not fully accessible, please specify the expected impact on services until access is fully restored.
Duration of a typical access (average) and number of external users expected for that access
2-6 weeks

Quality Control / Quality Assurance (QA)

Activities / tests / data are:
Controlled: ISO 9001:2015
Link to your institution QA webpages if available:
https://www.bgs.ac.uk/about/accred.html https://www.bgs.ac.uk/about/docs/BSI_FS_71346Certificate_2015.pdf

Operational or other constraints

Specific risks:
All risks associated with operating laboratory equipment are covered in the TPRL working protocols and associated risk assessments which are provided to laboratory users.
Legal issues:
nd

CCUS Projects

Other CCUS Projects
OTHER LARGE INITIATIVES
Microbial diversity and the effects of exposure to elevated CO2 on sediments from at a site in Niigata, Japan, in collaboration with colleagues from the Research Institute of Innovative Technology for the Earth (RITE).
EPSRC funded EngD studentship (Industrial Doctorate Centre in Carbon Capture and Storage and Cleaner Fossil Energy)
2014 - 2018
Mitigating the impact of microbes on the storage of carbon dioxide.
EU-Funded CCUS Projects
ERA-Net ACT: project no. 271498)
ELEGANCY (Enabling the Low carbon Economy by hydrogen and CCS)
ERA-Net ACT
REX-CO2 - Reusing existing wells for CO2 storage operations
H2020
SECURe (Subsurface evaluation of CCS and unconventional risks – methane oxidizing bacteria in groundwater)
Horizon 2020
MIND (Microbiology In Nuclear Disposal -EU project 661880)
Nd and HREE NERC project NE/M01116X/1
SoS RARE: (Multidisciplinary research towards a secure and environmentally sustainable supply of critical rare earth elements )

Selected Publications

Energy Procedia, 114, 3077-3087. (2017)
The effect of variable oxygen impurities on microbial activity in conditions resembling geological storage sites.
Morgan, H., Large, D., Bateman, K., Hanstock, D., & Gregory, S.
International Journal of Greenhouse Gas Control, 111, p.103479. (2021)
Potential impacts of oxygen impurities in carbon capture and storage on microbial community composition and activity.
Morgan, H., Large, D.J., Bateman, K., Hanstock, D. and Gregory, S.P.
Advances in Geo-Energy Research, 5(1), pp.5-7. (2021)
The relevance of microbial processes in geo-energy applications.
Ebigbo, A. and Gregory, S.P.,

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